No matter how high your defenses, I guarantee you’ll find at least one that breaks them down. I count at least ten stone-cold classics in her discography. (A carpetbagger from the Pennsylvania suburbs, she became an expert code-switcher early in her career and never looked back.) And when it comes to writing instantly memorable pop songs, her only peers are a few anonymous Swedish guys, none of whom perform their own stuff. She may not have the raw vocal power of some of her competitors, but what she lacks in Mariah-level range she makes up for in versatility and personality. Even with the widespread critical embrace of poptimism - a development I suspect has as much to do with the economics of online media as it does with the shifting winds of taste - there are still those who see Swift as just another industry widget, a Miley or Katy with the tuner set to “girl with a guitar.” If this list does anything, I hope it convinces you that, underneath all the think pieces, exes, and feuds, she is one of our era’s great singer-songwriters. So, uh, I don’t recommend you listen to this list top to bottom.īut I do recommend sampling as many of these songs as you see fit. What sort of real life can stand up against fantasies like these? Listen to too many and you might ache again at the nagging feeling that those stories of yours have all been a bit uneventful and drab by comparison. (This aspect of her songwriting matured somewhat with the coming of the Joe Alwyn era, as the love stories got slightly more quotidian, but even those were filled with secret affairs begun in dark bars - a mite more dramatic than Netflix and chill.) Listen to her songs and you’ll ache at the resemblance to the most dramatic moments in your own private history. An unworthy suitor wouldn’t just say something thoughtless he’d skip a birthday party, or leave a teenage girl crying alone in a hotel room. Instead, they showed up at each other’s doors late at night and they kissed in the rain. On her first five albums, her characters never eased into a relationship. Swift - or at least the version of Swift on her albums - has remained largely the same person since her debut: a thin-skinned, bighearted obsessive, with a penchant for huge romantic moments. Other people say she’s Jewish.Īnd yet, if the word on her has shifted since her debut, it’s because we’ve changed, not her. Some people say she’s a goddess of the alt-right. The world’s most famous serial dater, now settled into a long-term relationship with the guy from The Favourite. She’s been feminism’s worst nightmare, and an advocate for victims of sexual assault. She was a precocious teenager, and the ultimate embodiment of white privilege. And, outside the legions of fans who eat up everything she puts out, no take on her ever stays solid for long. For more than a decade, a 31-year-old woman from Wyomissing, Pennsylvania, has found herself at the center of our national conversations about race, gender, celebrity, victimhood, and even the intricacies of recording contract law. One of them is a massive, multimillion-dollar enterprise filled with violence and betrayal, and the other aired on HBO. In all my years in the business, there have been two subjects that could boost your page views like nothing else: Game of Thrones and Taylor Swift.
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Mutagenesis has gained popularity in plant genetics research in the last century as a powerful tool for inducing genetic variation. Rafael Garcés, in Sunflower, 2015 Publisher Summary mutagenesis and recombinogenesis.Īndrés Zambelli. The observation that unrepaired DNA damage also increases the cellular recombinogenic activity, leads us to speculate that SOS-induction might contribute to the adaptability (population fitness) through rapidly inducible mechanisms of genetic variability e.g. Unrepaired non-pairing DNA lesions cause SOS induction which would seem to result in reversible inhibition of the proof-reading activity, causing decreased replicational fidelity (“untargeted” mutagenesis) and an increased capacity to copy DNA lesions (“targeted” mutagenesis c.f. Such a 3′ to 5′ exonuclease with typical proof-reading activities has been isolated from mammalian cells. This is probably due to the polymerase-associated 3′ to 5′ exonuclease (proof-reading) activity. Non-pairing DNA lesions, usually bulky adducts to the bases, cannot be copied by the constitutive DNA replication enzymes either in vivo or in vitro, and therefore do not result in direct mutagenesis. Mis-pairing DNA bases, which are readily copied or incorporated into DNA, appear to be subject to a post-replicative methylation-instructed mismatch repair. Mutagenesis by mis-pairing and by non-pairing DNA lesions is reviewed. Radman, in DNA Repair Mechanisms, 1978 ABSTRACT Here, we describe these reporter systems, provide protocols for use, and demonstrate their key functional features using error-prone Pol I mutagenesis as a source of mutations. This reporter couples two reversion markers, allowing the temporal separation of mutation events in time, thus providing information about the dynamics of mutagenesis in mutator strains. We also describe a reporter aimed at facilitating the monitoring of continuous mutagenesis in mutator strains. This output is obtained by coupling a TEM β lactamase-based reversion assay with GFP fluorescence, either by placing the two genes on the same plasmid or by fusing them translationally and interrupting the N-terminus of the chimeric ORF with a stop codon. Here, we present two reversion reporters for in vivo mutagenesis that produce a quantitative output, and thus have the potential to greatly reduce the amount of test chemical and labor involved in these assays. Both methods are labor intensive, involving visual screening, quantification of colonies on solid media, or determining a Poisson distribution in liquid culture. This chapter focuses on detection of mutagenesis in prokaryotes, which boils down to two approaches: reporter inactivation (forward mutation assay) and reversion of an inactivating mutation (reversion mutation assay). Mutagenesis assays are also used to study mechanisms of DNA homeostasis. Mutagenesis in model organisms following exposure to chemicals is used as an indicator of genotoxicity. Manel Camps, in Methods in Enzymology, 2017 Abstract c Includes insecticides (organochlorines, organophosphates, carbamates, pyrethroids), herbicides, and fungicides. b Oxidative damage includes oxidized bases, change in chemical structure of bases (e.g., open rings), strand breaks, base loss, DNA–protein adducts, and lipid aldehyde adducts. Oxidative damage, a, b base loss and fragmentation, DNA–DNA cross-linksįormation of oxyradicals from water and oxygenĭirect interaction of radioactive particle with DNA sugars and basesĪ Adducts and oxidized bases may lead to production of abasic sites via destabilization of the glycoside (sugar base) linkage. Uranium ore mining and fuel processing, nuclear energy, nuclear weapons, combustion of coal Interference with mitochondrial metabolism Industrial manufacturing, agricultural chemicals, ore mining and smelting, steel manufacture, building materials and paints, gasoline additives, fossil fuel extraction, combustion of coal, battery manufacture and disposal, metal plating, photographic emulsions, paper manufacture Transition metals, heavy metals, and arsenic Industrial manufacturing, paper processing, electrical insulators, cleaning and degreasing agents, solvents, chemical industry, combustion and manufacture of plastics, flame retardants, stain repellentsĪgricultural, commercial, and residential applications Halogenated organics (PCBs, dioxins, chlorinated solvents, perfluorocarbons, brominated aromatic hydrocarbons) Combustion of organic matter and fossil fuels, crude oil and coal spills and leaching, copier toner cartridges, coal coking, creosote, used oil and lubricants, asphalt
Though he is a malak (a spiritual being that can harness the power of nature), he sails the Van Eltia pirate ship on the open seas to follow his own creed. Introducing the Eleanor Pirate Preator Outfit This texture re-colour will make Eleanors Pirate Outfit look like she altered her Exorcist outfits. Yea, the Pirate ones are awesome, really think Magilous best is the Idol with a Witch Hat or the Maid one, havent bought Maid or School outfits though, only really like Magilous for the maid set and only Rokurous for the school set. While looking for the missing Captain Van Aifread, he meets Velvet and joins her to fight their common enemy, the Abbey.Ĭonversely, Eleanor defends the Abbey against Velvet’s repeated attacks. Eleanor Hume, also known as Eleanor, is a main character in Tales of Berseria. Actually thats really the problem only Phi and Eleanor have dull default outfits. Tales of Berseria will have paid DLC costumes that feature summer-style. She is a praetor rank Exorcist belonging to Abbey. examples shown here are Eizen (summer), Eleanor (Japanese) and Velvet (pirate). She travels the world with the sole mission of cleansing the world from Daemons. The Pirate outfits just seem to fit the tone of the game a lot more than the defaults. As a praetor, Eleanor always scrabbles to release the world from daemons threat, but fate will eventually make her join Velvet’s party. Darkdragon2335 5 years ago 2 I'm not a big fan of Laphicet and Eizen's costumes, but Magilou and Rokurou really look like they belong in those piratey clothes. The young girl tries to be reasonable as much as possible but she can sometimes be overcome by emotion, because she is honest with a great compassion for others.īandai Namco also talked about the skit system, which introduces a “cut-in” effect in Tales of Berseria, where character illustrations are inserted in various ways depending on their conversation. “Sometimes a skit describes a single character’s facial expression, sometimes it shows a conversation among multiple characters,” they added. “Expressions really change depending on the cut.”Īs for the Switch Blast battle system, Bandai Namco explained that it allows you to switch characters during battle and choose a back-up character to replace someone in the battle. Recolored outfit (White or Dark version) Pants restored. The back-up character has one more soul than usual, and each character has a unique attack when they join the battle through Switch Blast. Recommend putting the eyepatch accessory if you have it in-game. Try it now and let us know what you think! We are always improving. This is the coolest Euchre card game ever. I have referred EUCHRE 3D to at least a dozen other players and all thanked me for how it improved their game and enjoyment. IMHO the reviewer is just plain untruthful. I am considered a very good player and have been high and score many times in many different location games of 24-76 players. Enjoy realistic graphics and smooth gameplay Get instant help anytime you get stuck with in-game advice. EUCHRE 3D has been my main learning tool. Online multiplayer keeps gameplay fresh Your game, your way: choose from different game styles and speeds. Opponents that self adjust to your playing level Play with or against the computer to improve your skills and win exciting achievements. With big and beautiful cards, a clear interface, hand picked sounds and an amazing Artificial Intelligence, Euchre, the Classic Card Game, offers you an experience like no other before. This will be your best ever experience on an Euchre game! The primary rule to remember when playing Euchre is that one is never required to play the trump suit (unless that is the one that is led), but one is required to follow suit if possible.Įnjoy the essence of the classic Euchre game, specially conceived for your Android. If euchred while playing alone, the opposing team still only receives 2 points. If only three or four of the tricks are taken while going alone, then only one point is scored. The partner of a caller in a lone hand does not play, and if the caller achieves the march (winning all 5 tricks), the winning partners score 4 points. Euchre Score Sheets: Euchre Score Book For Tracking Your Favorite Game Euchre Score Sheet Farkle Score Card Large Print 8.5 x 11 120 Score pages. A failure of the team to win three tricks is referred to as being "euchred", and that team is penalized by giving the opposing team two points.Ī caller with very good cards can "go alone" (order up alone), in which case the caller tries to win the march without a partner. A single point is scored if the team takes three or four tricks, and two points are scored if the team takes all five tricks. When naming a suit (ordering up trump), a player states that their team intends to win the majority of tricks of the hand. Get the best card game ever: Euchre! It is simple, fast to learn and offers you guaranteed fun!Įuchre is played with a deck of 24 standard playing cards. Euchre 3D is the 1 free Euchre card game for iPhone and iPad Euchre 3D has been in development for years by a dedicated team and has seen countless new features, improvements, and bug fixes. ***** "Can't think of anything I don't like.Join millions of fanatical Euchre players. Just like the four handed with real people." ***** "Very addictive, and the AI is surprisingly realistic." Plays quick, plays fair, and is just tough enough to keep me coming back for more! Highly recommended for the Euchre fan!" ***** "This game has absorbed my time more than the words with friends game. This app is identical to our free app but does not contain any ads. choosing between accepting the trump, passing or changing your cards. * Frequent updates, improvements, and bug fixes In our trickster Euchre free app, you play with 3d ifferent other players Euchre. * In-app help and feedback menu (let us know how we can improve) * Game options, including Canadian Loner, Stick the Dealer, and more Click to find the best Results for euchre score Models for your 3D Printer. * Realistic graphics - it feels just like sitting at a table! Every Day new 3D Models from all over the World. * Fast, smooth playing with different game speeds Euchre 3D is the 1 Euchre card game for iPhone and iPad Euchre 3D has been in development for years by a dedicated team and has seen countless new features, improvements, and bug fixes. * Smart AI partners and opponents to keep you challenged It's fast, stable, always improving, and, above all, fun! #1 Euchre card game! Multiplayer or Solo Įuchre 3D is the #1 Euchre card game for iPhone and iPad! Euchre 3D has been in development for years by a dedicated team and has seen countless new features, improvements, and bug fixes. The Basel event types provide a central point of comparison, as they are universally adopted, have longevity and provide a basis for the ways in which institutions share data and provide insights into operational risks. Constructing a mutually exclusive and collectively exhaustive taxonomy driven by data is not without its challenges, and the careful narrowing of scope and separation of risks, in partnership with industry experts, was sometimes needed. As part of this work, we look at some of the contemporary concerns that have shaped how institutions think about their risks. The resultant taxonomy is a two-layered hierarchy of risks, with sixteen risks at level 1 and sixty-one risks at level 2. This combines both the theoretical and the practical wisdom of the crowd. In this paper, we take a data-driven approach and combine the individual active taxonomies of sixty large financial institutions (fifty-eight for construction and two for validation) to create a coherent new reference taxonomy: the ORX reference taxonomy for operational and nonfinancial risk. In doing so, they are deviating from Basel Committee on Banking Supervision event types, but without a common standard for which to aim. This changing risk profile, combined with a recent shift of focus away from capital measurement toward risk management, means that many organizations are actively revising their operational risk taxonomies. How organizations think about this expanding portfolio of threats and manage them in a consistent way is underpinned by their risk taxonomy. Risks such as conduct, cyber and third party have risen in importance and now dominate boardroom agendas. There has been a substantial change in the operational risks faced by financial services firms over the last fifteen years. Reset the Core Audio API #Ĭore Audio is a low-level API that deals with sound in Apple’s macOS and iOS. If, despite performing all of these trivial fixes, the volume icon remains grayed out, or you don’t hear sound from your built-in internal speakers, move on to other fixes. To rule out its possibility and ensure this isn’t causing sound issues on your Mac, open the browser and make sure the tiny speaker icon in the tab playing audio is unmuted.Īlternatively, you can also try playing audio in other apps to see if there’s sound. It usually occurs in browsers with the mute feature, which lets you mute a specific tab in the browser. Check if the Sound Is Working on Other Apps #Īlthough uncommon, there are times when you may not hear sound in a particular app on your Mac. Once that’s done, test your speaker by playing some audio. And then, make sure your built-in speakers are set as the output source by clicking on the volume icon in the menu bar and selecting Internal Speakers under Sound. If you’ve started experiencing sound issues after switching output devices, you must, first, disconnect all audio accessories connected to your Mac. Reconnect Audio Devices and Choose the Right Output Device #Ī lot of the time, connecting a defective audio accessory, leaving an audio accessory plugged-in to your Mac for an extended period, or abruptly connecting/disconnecting an audio device during playback can also cause sound issues on Mac. Here, tap on the Output tab and make sure the Mute option is unchecked. If your Mac’s audio is muted, pressing any of these keys should unmute it, and you should be able to hear the audio again.Īnother way to check if your Mac’s audio is on mute is through the device settings. Alternatively, you can click on the volume control in the menu bar and move the output volume slider to do this. Check Mac Volume #įirst off, check if your Mac’s system volume hasn’t been muted accidentally.įor this, press any of the volume buttons on your keyboard (F10 (mute/unmute), F11 (volume down), F12 (volume up)) to see if you’re able to change the system volume. Of course, if you’ve been a Mac user for some time, chances are you’ve probably already tried these fixes, in which case, you can skip to the next solution. Trivial Fixes for Mac Sound Not Working Issue #īefore you embark on the complex sound troubleshooting process, we’d recommend you try out a few trivial fixes to resolve no sound issues on your Mac. Here’s a list of such solutions we find effective in troubleshooting Mac’s sound not working issue. If you’re experiencing a sound glitch on your Mac, wherein there’s no audio from internal speakers and increasing/decreasing volume isn’t helping either, there are some really effective fixes that can help you fix the audio problem on your Mac. You may run into them when you connect or unplug an audio accessory during playback, use an app that accidentally misconfigures sound settings in the background, update to a buggy macOS version, or sometimes, for no apparent reason at all. If you would like more information on how to manage the settings of your Mac, please drop into the store and one of our knowledgeable staff will be more than happy to assist you.Sound issues on Mac are pretty common. You will now notice that annoying beep, beep, beep is gone forever. Simply remove the tick from the box and close the Preferences. By default the option for ‘Play Feedback When Volume Is Changed’ will be active. To permanently disable this disturbing sound, proceed to System Preferences > Sound then click on the tab for Sound Effects. To temporarily turn it off, hold down the Shift key as you increase, or decrease, the volume. Turn Off The Beep Sound When Increasing, Or Decreasing The Volume On Your Mac:ĭoes that beep, beep, beep noise when increasing, or decreasing, the volume on your Mac drive you insane? To solve this and thereby increase, or decrease, the volume by smaller step increments simply press Option + Shift + Volume Up, or Volume Down keys.Īs you can see from the image above, the onscreen volume guide will visually show these smaller increments. Sometimes this results in the level of the volume being either too high, or too low, for your specific requirements. When you increase, or decrease, the volume on your Mac, you may have noticed that it will be done in rather large step increments. Increase, Or Decrease, The Volume On Your Mac In Quarter Increments: Today we are going to look at two options you may be unaware of for managing the volume output of your Mac. So, don't worry when buying Abrosoft FantaMorph Deluxe for Windows with our offering coupon code.Ībrosoft FantaMorph Deluxe for Windows is backed by Fantamorph's guarantee of quality customer service. ShareIT/2Checkout/Cleverbridge/Payproglobal support Credit/Debit Card, PayPal and 45+ other payment methods. 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As this is still a construction step, we are not drawing the mane in any detail. Many of you will not have drawn zebras manes before, but it is not very difficult at all. Step 6: Drawing the Mane of Your Zebra Art Just before you reach the main body oval, create a little hump to represent the chest of our zebra drawings. For the bottom neckline, you want to begin just below the midpoint of the head oval and take the line down towards the bottom of the main body oval. Begin with the upper neckline, creating a smooth curve from the top point of the head oval to the top of the main body oval. You are going to use these two lines to join the head and body of your zebra drawing. You are now going to bring your zebra art to life a little more by drawing in the necklines. Step 5: Creating the Neck of Your Zebra Drawing The placement of this ear is important, as it should be placed at the highest point in the head oval.ĭraw a pointy ear that begins just within the head oval. Step 4: Outlining the Ear of Your Zebra Drawingĭue to the perspective that our zebra drawings are from, only one of the ears is visible. You want the bottom side of this muzzle to curve up slightly before it touches back onto the head oval. To lay down the shape of the muzzle, you are going to draw an uneven “U” shaped curve on the end of the head oval shape. Things start to get a little more freehand in this step. Step 3: Creating the Muzzle of Your Zebra Drawing Place this small tilted head oval some space away from the main body oval to the left. This head oval should be much smaller than the body oval and it should be tilted with the top leaning towards the back of the canvas. Remember to place the main body oval in the very center of your canvas leaving plenty of space on all sides for the other parts of the zebra’s body.įor the head shape outline, you are going to use another oval shape. Draw this oval laying horizontally on your canvas. The best shape for the main body is a large oval shape. To begin our zebra drawings, we are going to use a construction shape to lay down the foundations for the main body of the zebra. Step 1: Creating the Shape of the Main Body So, whether it is acrylic paint or coloring pencils, gather your drawing supplies, and let us begin our realistic zebra drawing. We have used a graphic tablet for our example drawings, but you can adjust the coloring steps to suit your chosen medium. The medium that you choose for these coloring steps is completely up to you. Take a look at the collage below to see an outline of the zebra drawing tutorial.Īlthough zebras are black and white, you will need to add color in the last few steps. This is just one of the ways that we make learning how to draw a zebra easy. This is why we begin our tutorial with eight construction steps to help you lay down the shape of the zebra drawing before you begin adding details and coloring. When you first approach a zebra drawing, you may feel a little overwhelmed. How to Draw a Zebra Step-by-Step Tutorial 3.1 If You Have Not Drawn Zebras Before Will This Tutorial Help?.2 Video Tutorial on How to Draw a Zebra.1.18 Step 18: Finishing Our Zebra Drawings.1.17 Step 17: Creating Highlights Within the Pattern.1.16 Step 16: Adding Color to the Zebra Art Pattern.1.15 Step 15: Adding Complimentary Highlights to Your Zebra Drawing.1.14 Step 14: More Shading and Detail Coloring.1.13 Step 13: Creating Some Light Shading.1.12 Step 12: Applying the Base Color Coat.1.11 Step 11: Creating the Zebra Art Patterns.1.9 Step 9: How to Draw a Zebra Easy Outline.1.8 Step 8: Shaping the Rump and Back Legs of Your Zebra Drawing.1.7 Step 7: Outlining the Shape of the Front Legs.1.6 Step 6: Drawing the Mane of Your Zebra Art.1.5 Step 5: Creating the Neck of Your Zebra Drawing.1.4 Step 4: Outlining the Ear of Your Zebra Drawing.1.3 Step 3: Creating the Muzzle of Your Zebra Drawing.1.1 Step 1: Creating the Shape of the Main Body.1 How to Draw a Zebra Step-by-Step Tutorial. Th last great boa-defeating secret is white vinegar, which for some reason they can’t stand. Boa constrictors also find hot water and cold water intolerable – any difference to their warm and humid norm in the jungle. If you’re bathing in the candlelight, then simply chuck your red wine glass in its face. The alcohol should be placed in front of the boa’s nostrils, and the result will be an instant release in pressure. Boa constrictors hate the taste and scent of alcohol, and professional snake handlers commonly keep a bottle and cotton swabs close at hand. Should you ever wake up to find a boa constrictor coiled around your neck, there’s a simple strategy: grab your unfinished can of beer. © Wikimedia Commons User: Jose Reynaldo da Fonseca / CC BY-SA-3.0 They actually use asphyxiation, cutting off the blood flow to the creature’s brain and rendering it unconscious within seconds. One misconception is that boa constrictors suffocate their prey to death. 25psi is nothing compared to a saltwater crocodile’s 3700psi, but the boa constrictor uses its entire 2.5 metre body. Finally, the boa constrictor will squeeze with all its body weight. With its hooked teeth holding the prey in place, the boa constrictor instantly uses its remaining momentum to coil itself around the poor creature’s neck. When a rodent or lizard finally saunters past, the boa constrictor will propel itself from its secure branch perch with immense speed, and bite the prey deeply and accurately. Only rarely will a boa constrictor actively stalk its prey its time-tested strategy is the ambush. It will occasionally dangle its head down while flecking its tongue to analyse its surroundings, before retreating upwards to the shadows. The boa constrictor begins the hunt by slithering up a tree and waiting in place patiently. On the outskirts of the Amazon rainforest, the boa constrictor’s diet includes rodents, mice, bats and even monkeys. © Wikimedia Commons User: Tiago Falótico / CC BY-SA-4.0 Boa constrictors commonly grip handlers’ arms in snake enclosures, causing a spike of fear in the victim, but this is actually to prevent themselves from plummeting to the hard floor. One common misconception is that boa constrictors attack human beings at the drop of a hat, but in reality, they’re rarely aggressive. The species only measures 1.2 metres compared to the boa constrictor’s average of 2.0, yet the kingsnake has been witnessed taking on snakes much larger than itself, including the 1.5 metre ratsnake. However, it’s the California kingsnake which has the highest squeeze force relative to its size. By comparison, the average Burmese python, reticulated python, and ball python score 6.23, 7.2, and 4.0 respectively. This increased 2.6 fold when the diameter of the snake was doubled. The boa constrictor has the highest pounds per square inch squeeze force of any surviving snake, reaching 25PSI in a 2007 study. © Wikimedia Commons User: Angela Rothermann / CC BY-SA-3.0Įveryone knows that boa constrictors dispatch their prey via the cuddle of a lifetime, and science has now proven that the hype is justified. Scientists have no idea how many boa constrictors exist in the world, let alone whether they’re rising or falling. Being so common, their population numbers are a complete mystery. They particularly favour rainforests and grassy clearings within them, but woodlands, grasslands, dry tropical forest, thorn scrub, and semi-desert are also popular hangouts. The boa constrictor can thrive in almost any habitat. The only South American country they don’t inhabit is Chile, because of the impenetrable high Andes mountain range along its eastern border. The species also defies the ocean, and has natural populations on several islands, such as Lesser Antilles, Trinidad, Tobago, Dominica and St Lucia. Their southern reaches extend deep in Argentina, while their northern flanks just cross the border into Panama. Boa constrictors can be found slithering over approximately 2 thirds of the continent, including Brazil, Columbia, Venezuela, Paraguay, Bolivia, and more. The boa constrictor is an icon of panic, and making matters worse, it’s extremely common in its native South America. © Wikimedia Commons User: Gionorossi / CC BY-SA-4.0įew names in the snake world inspire fear quite like the boa constrictor, except maybe the black mamba (particularly since Kill Bill Volume 2 was released in 2004). |
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